The dataset, titled "A queen odour mediates reproductive suppression in a eusocial mammal," version 3.0, contains raw data for the associated publication in the subject of Biology. It includes codes such as MATLAB source code for NMR_cfos_DoG_localization, a T maze MATLAB code, and an R syntax file for XCMS CAMERA_package, alongside extensive immunofluorescence data files (e.g., Experiment-2248.czi through Experiment-2294.czi). The dataset's purpose is to provide the underlying experimental data supporting the study on how queen odour mediates reproductive suppression in a eusocial mammal, available under a CC0 1.0 license.