The dataset describes the Spelunker platform, the latest Neuroglancer version for proofreading and annotating large electron microscopy datasets using CAVE. It contains a user interface with 2D and 3D segmentation layers, mouse and keyboard navigation controls, and tools for manipulating segment IDs, splitting, merging, finding paths, and adding annotations. The purpose is to guide both new and experienced users in proofreading and fixing neurons within large EM image volumes, with features for identifying natural branch terminations and locating cells via coordinates or segment IDs.