The dataset comprises reference data from transgenic characterization studies focused on neural projections, utilizing BDA (biotinylated dextran amine) and AAV (adeno-associated virus) tracing techniques. It contains detailed anatomical and molecular information derived from experimental comparisons of neural circuitry in model organisms, likely mice, with applications in neuroscience research. The data supports analysis of axonal pathways, synaptic connectivity, and genetic markers associated with specific brain regions, serving as a resource for understanding neuroanatomy and functional connectivity. Integrated within the Brain Explorer platform, it aids researchers in mapping and comparing neural networks, facilitating studies on brain development, disease mechanisms, and therapeutic targeting. The dataset is structured to enable high-resolution imaging and quantitative analysis of transgenic markers, enhancing precision in neurobiological investigations.